Molecular pathology in the lungs of severe acute respiratory syndrome patients.
Identifieur interne : 003655 ( Main/Exploration ); précédent : 003654; suivant : 003656Molecular pathology in the lungs of severe acute respiratory syndrome patients.
Auteurs : Juxiang Ye [République populaire de Chine] ; Bo Zhang ; Jian Xu ; Qing Chang ; Michael A. Mcnutt ; Christine Korteweg ; Encong Gong ; Jiang GuSource :
- The American journal of pathology [ 0002-9440 ] ; 2007.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Cellules épithéliales (anatomopathologie), Cellules épithéliales (virologie), Femelle, Fibroblastes (anatomopathologie), Fibroblastes (virologie), Humains, Hybridation in situ, Immunohistochimie, Lymphocytes T (anatomopathologie), Lymphocytes T (virologie), Macrophages alvéolaires (anatomopathologie), Macrophages alvéolaires (virologie), Mâle, Poumon (anatomopathologie), Poumon (virologie), Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS.
- MESH :
- anatomopathologie : Cellules épithéliales, Fibroblastes, Lymphocytes T, Macrophages alvéolaires, Poumon, Syndrome respiratoire aigu sévère.
- virologie : Cellules épithéliales, Fibroblastes, Lymphocytes T, Macrophages alvéolaires, Poumon, Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Femelle, Humains, Hybridation in situ, Immunohistochimie, Mâle, Virus du SRAS.
English descriptors
- KwdEn :
- Adult, Epithelial Cells (pathology), Epithelial Cells (virology), Female, Fibroblasts (pathology), Fibroblasts (virology), Humans, Immunohistochemistry, In Situ Hybridization, Lung (pathology), Lung (virology), Macrophages, Alveolar (pathology), Macrophages, Alveolar (virology), Male, Middle Aged, SARS Virus, Severe Acute Respiratory Syndrome (pathology), Severe Acute Respiratory Syndrome (virology), T-Lymphocytes (pathology), T-Lymphocytes (virology).
- MESH :
- pathology : Epithelial Cells, Fibroblasts, Lung, Macrophages, Alveolar, Severe Acute Respiratory Syndrome, T-Lymphocytes.
- virology : Epithelial Cells, Fibroblasts, Lung, Macrophages, Alveolar, Severe Acute Respiratory Syndrome, T-Lymphocytes.
- Adult, Female, Humans, Immunohistochemistry, In Situ Hybridization, Male, Middle Aged, SARS Virus.
Abstract
Severe acute respiratory syndrome (SARS) is a novel infectious disease with disastrous clinical consequences, in which the lungs are the major target organs. Previous studies have described the general pathology in the lungs of SARS patients and have identified some of the cell types infected by SARS coronavirus (SARS-CoV). However, at the time of this writing, there were no comprehensive reports of the cellular distribution of the virus in lung tissue. In this study, we have performed double labeling combining in situ hybridization with immunohistochemistry and alternating each of these techniques separately in consecutive sections to evaluate the viral distribution on various cell types in the lungs of seven patients affected with SARS. We found that SARS-CoV was present in bronchial epithelium, type I and II pneumocytes, T lymphocytes, and macrophages/monocytes. For pneumocytes, T lymphocytes, and macrophages, the infection rates were calculated. In addition, our present study is the first to demonstrate infection of endothelial cells and fibroblasts in SARS.
DOI: 10.2353/ajpath.2007.060469
PubMed: 17255322
Affiliations:
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Mcnutt</name></author><author><name sortKey="Korteweg, Christine" sort="Korteweg, Christine" uniqKey="Korteweg C" first="Christine" last="Korteweg">Christine Korteweg</name></author><author><name sortKey="Gong, Encong" sort="Gong, Encong" uniqKey="Gong E" first="Encong" last="Gong">Encong Gong</name></author><author><name sortKey="Gu, Jiang" sort="Gu, Jiang" uniqKey="Gu J" first="Jiang" last="Gu">Jiang Gu</name></author></analytic><series><title level="j">The American journal of pathology</title><idno type="ISSN">0002-9440</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Epithelial Cells (pathology)</term><term>Epithelial Cells (virology)</term><term>Female</term><term>Fibroblasts (pathology)</term><term>Fibroblasts (virology)</term><term>Humans</term><term>Immunohistochemistry</term><term>In Situ Hybridization</term><term>Lung (pathology)</term><term>Lung (virology)</term><term>Macrophages, Alveolar (pathology)</term><term>Macrophages, Alveolar (virology)</term><term>Male</term><term>Middle Aged</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome (pathology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>T-Lymphocytes (pathology)</term><term>T-Lymphocytes (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Cellules épithéliales (anatomopathologie)</term><term>Cellules épithéliales (virologie)</term><term>Femelle</term><term>Fibroblastes (anatomopathologie)</term><term>Fibroblastes (virologie)</term><term>Humains</term><term>Hybridation in situ</term><term>Immunohistochimie</term><term>Lymphocytes T (anatomopathologie)</term><term>Lymphocytes T (virologie)</term><term>Macrophages alvéolaires (anatomopathologie)</term><term>Macrophages alvéolaires (virologie)</term><term>Mâle</term><term>Poumon (anatomopathologie)</term><term>Poumon (virologie)</term><term>Syndrome respiratoire aigu sévère (anatomopathologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Cellules épithéliales</term><term>Fibroblastes</term><term>Lymphocytes T</term><term>Macrophages alvéolaires</term><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Epithelial Cells</term><term>Fibroblasts</term><term>Lung</term><term>Macrophages, Alveolar</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term><term>Fibroblastes</term><term>Lymphocytes T</term><term>Macrophages alvéolaires</term><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term><term>Fibroblasts</term><term>Lung</term><term>Macrophages, Alveolar</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>In Situ Hybridization</term><term>Male</term><term>Middle Aged</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Femelle</term><term>Humains</term><term>Hybridation in situ</term><term>Immunohistochimie</term><term>Mâle</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a novel infectious disease with disastrous clinical consequences, in which the lungs are the major target organs. Previous studies have described the general pathology in the lungs of SARS patients and have identified some of the cell types infected by SARS coronavirus (SARS-CoV). However, at the time of this writing, there were no comprehensive reports of the cellular distribution of the virus in lung tissue. In this study, we have performed double labeling combining in situ hybridization with immunohistochemistry and alternating each of these techniques separately in consecutive sections to evaluate the viral distribution on various cell types in the lungs of seven patients affected with SARS. We found that SARS-CoV was present in bronchial epithelium, type I and II pneumocytes, T lymphocytes, and macrophages/monocytes. For pneumocytes, T lymphocytes, and macrophages, the infection rates were calculated. In addition, our present study is the first to demonstrate infection of endothelial cells and fibroblasts in SARS.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chang, Qing" sort="Chang, Qing" uniqKey="Chang Q" first="Qing" last="Chang">Qing Chang</name><name sortKey="Gong, Encong" sort="Gong, Encong" uniqKey="Gong E" first="Encong" last="Gong">Encong Gong</name><name sortKey="Gu, Jiang" sort="Gu, Jiang" uniqKey="Gu J" first="Jiang" last="Gu">Jiang Gu</name><name sortKey="Korteweg, Christine" sort="Korteweg, Christine" uniqKey="Korteweg C" first="Christine" last="Korteweg">Christine Korteweg</name><name sortKey="Mcnutt, Michael A" sort="Mcnutt, Michael A" uniqKey="Mcnutt M" first="Michael A" last="Mcnutt">Michael A. Mcnutt</name><name sortKey="Xu, Jian" sort="Xu, Jian" uniqKey="Xu J" first="Jian" last="Xu">Jian Xu</name><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Ye, Juxiang" sort="Ye, Juxiang" uniqKey="Ye J" first="Juxiang" last="Ye">Juxiang Ye</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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